· Front page
· Partners
· Objectives
· Methodology
· Project Workplan
· Background of the proposal
· Expected benefits and achievements
· Contribution
· Innovation Aspects
· Results
· Exploitation and dissemination activities
· Conclusions
· News
· Duration of the project
· Contact
· Acknowledgements
· Work after the project was closed

Work after the project was closed

After closing this project, a new consortium continued the collaboration in the new EU-project, in order to further improve the diagnosis of viral diseases in animals and in animal products.

“New  and Emerging Technologies: Improved Laboratory and On-Site Detection of OIE List A Viruses in Animals and Animal Products”

(SSPE-CT-2004-513645). Acronym: LAB-ON-SITE

For more information see the web page of the LAB-ON-SITE project:

http://www.labonsite.com/

The members of the new consortium are:

Partner  1
The National Veterinary Institute (NVI), Uppsala, Sweden

Partner 2
Queen's University of Belfast (QUB), Virology Laboratory, Veterinary Sciences Department (VSD), Northern Ireland, UK.

Partner 3
Danish Institute for Food and Veterinary Research (DFVF), Department  of Virology, Lindholm, Denmark

Partner 4
Universidad Complutense de Madrid (UCM), Facultad de Veterinaria, Madrid, Spain

Partner 5
Veterinary Institute of Debrecen (VID), Debrecen, Hungary

Partner 6
SVANOVA Biotech AB, Uppsala, Sweden

Partner  7
Istituto Zooprofilattico Sperimentale della Lombardia e dell´Emilia  Romagna (IZSLER), Brescia, Italy

Partner 8
Ghent University (UGENT), Merelbeke, Belgium

Partner 9
The Institute for Animal Health (IAH), Pirbright, United Kingdom

Subcontractor to P1
OLINK AB, Uppsala, Sweden

Subcontractor to P4
The Research Center Borstel (RCB), Leibnitz-Center, Center for Medicine and Biosciences, the Division of Veterinary Infectiology and Immunology, Germany.

Third party
FAO/IAEA Joint Division, IAEA, Vienna, Austria

Disseminating the knowledge after this project was closed and the work is continued in the LAB-ON-SITE project, with the involvement of three new partners
http://www.labonsite.com/

Year 2005
Seminars and oral presentations
Hakhverdyan M., Belák S. (2005). Experiences on the application of various nucleic acid amplification systems for the improved of detection of vesicular diseases. New Diagnostic Technology: Applications in Animal Health & Biologics Controls. Applications in disease surveillance, molecular epidemiology and quality control tests of vaccines. 3-5 October 2005, Saint-Malo – France. Oral presentation.
German P. (2005). Detection of Avian influenza virus and Newcastle disease virus by a novel real-time RT-PCR using light upon extension (LUX) fluorogenic primers. Annual Meeting of the Hungarian Society for Microbiology, jointly organized with the 1st Central European Forum for Microbiology (CEFORM). October 26th-28th 2005, Keszthely, Hungary. Oral presentation.
Uttenthal Å, Rasmussen TB, Thorén P, Belák S (2005). The standardisation, quality control and validation of molecular diagnostic tests. 7th OIE/WAVLD Seminar on Application of Biotechnology to Zoonotic Disease Diagnosis, 17th November 2005, Montevideo, Uruguay. Oral presentation.

Publications, Abstracts, Proceedings
Hakhverdyan M., Rasmussen T. B, Uttenthal Å., Thorén P., Belák S. (2005). Experiences on the application of various nucleic acid amplification systems for the improved of detection of swine vesicular disease virus. New Diagnostic Technology: Applications in Animal Health & Biologics Controls. Applications in disease surveillance, molecular epidemiology and quality control tests of vaccines. 3-5 October 2005, Saint-Malo – France. Proceedings, p. 4.
Hakhverdyan M, Rasmussen TB, Uttenthal Å, Belák S. (2005). Detection of swine vesicular disease virus (SVDV) using PriProET Real-Time PCR assay. IUMS, XIII International Congress of Virology, San Francisco, CA, USA, July 23-28, 2005:140-141.
Hjertner B.,Meehan B., McKillen J., McNeilly F., Belák S. (2005). Development and evaluation of an INVADER assay for the detection of African swine fever DNA. New Diagnostic Technology: Applications in Animal Health & Biologics Controls. Applications in disease surveillance, molecular epidemiology and quality control tests of vaccines. 3-5 October 2005, Saint-Malo – France. P. 80.
McKillen J., Brawley A., Hjertner B., McNeilly F., Allan G. (2005). Development of molecular beacon assay for rapid real-time polymerase chain reaction detection of four economically important viruses of swine. New Diagnostic Technology: Applications in Animal Health & Biologics Controls. Applications in disease surveillance, molecular epidemiology and quality control tests of vaccines. 3-5 October 2005, Saint-Malo – France. P. 86.
Rasmussen TB (2005): Molecular detection of exotic viral infections in animals using quantitative real-time RT-PCR. PhD-thesis, Royal Veterinary and Agricultural University, October 2005, Copenhagen, Denmark.

Year 2006
Seminars and oral presentations
Hjertner B, McMenamy M (2006). Isothermal detection methods in diagnostics: A look at loop-mediated isothermal amplification (LAMP). Oral presentation on annual ID-RRG (Infectious diseases recognised research group) away day 20-21 October 2006 (Ballycastle, NI).
Rasmussen TB, Uttenthal Å (2006). Validated CSFV and BVDV TaqMan RT-PCRs for combined pestivirus diagnostics. Oral presentation and report on Annual Meeting of National Classical Swine Fever Laboratories, Brussels.
Grazioli S, Moretti M, Barbieri I, Crosatti M, Brocchi E. (2006). Use of monoclonal antibodies to identify and map new antigenic determinants involved in neutralisation on FMD viruses type SAT 1 and SAT 2. Report of the Sess. Res. Gr. St. Tech. Committee of the EUFMD Commission. Oral presentationand submitted paper.
Grazioli S, Moretti M, Gamba D., Bugnetti M., Brocchi E. (2006). Monoclonal antibody-based multiplex ELISA for the detection and typing of the seven serotypes of FMDV. Poster presentation.

Publications, Abstracts, Proceedings
Antal, M., Farkas, T., Germán, P., Belák, S., Kiss, I. (2006): Real-time RT-PCR detection of Newcastle disease viruses by using LUX (light upon extension) fluorogenic primers. J Vet Diagnostic Investigation. Submitted
Banér J., Gyarmati P., Yacoub A., Hakhverdyan M., Belák S. (2006). Microarray-based molecular detection of foot-and-mouth disease virus, vesicular stomatitis virus and swine vesicular disease virus using padlock probes. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 220.
Banér J., Gyarmati P., Yacoub A., Hakhverdyan M., Stenberg J., Ericsson O., Nilsson M., Landegren U., Belák S. (2006). Microarray-based molecular detection of foot-and-mouth disease virus, vesicular stomatitis virus and swine vesicular disease virus, using padlock probes. J. Virol. Methods. Submitted.
Belák S., Hakhverdyan M. (2006). Recent achievements and trends in the molecular diagnosis of bovine viral diseases: a view from the “OIE Collaborating Centre for the Application of Polymerase Chain Reaction Methods for the Diagnosis of Viral Diseases in Veterinary Medicine”. Dtsch. Tierärztl. Wschr. 2006. 113(4):129-33. Review.
Cordioli P.; Moreno A., Lelli D., Sozzi E., Luppi A., Tranquillo V., Brocchi E., (2006). Monoclonal antibody-based elisas for the detection of antibodies against neuraminidase subtypes 1, 2 and 3 of influenza viruses. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 40.
Cordioli P.; Moreno A., Lelli D., Sozzi E., Gamba D., Brocchi E., (2006). Antigen typing elisas for h5 and h7 avian influenza viruses using monoclonal antibodies. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 195.
Grazioli S, Moretti M, Barbieri I, Crosatti M, Brocchi E. (2006). Use of monoclonal antibodies to identify and map new antigenic determinants involved in neutralisation on FMD viruses type SAT 1 and SAT 2. Report of the Sess. Res. Gr. St. Tech. Committee of the EUFMD Commission. Submitted.
Hakhverdyan M., Rasmussen T. B, Thorén P., Uttenthal Å., Belák S. (2006).  Development of a real-time PCR assay based on Primer-Probe Energy Transfer for the detection of swine vesicular disease virus. Arch Virol. 151 (12): 2365-2376.
Hakhverdyan M., Rasmussen T. B, Thorén P., Uttenthal Å., Belák S. (2006). Comparison between molecular beacon and PriProET real-time PCR assays based on detection ability of different SVDV strains. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 219.
Ebert K, King DP, Reid SM, Ferris NP. (2006). Comparison of RNA extraction Methods for RT-PCR Diagnosis of FMD. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 226.
Farkas, T., Antal, M., Sámi, L., Germán, P., Kecskeméti, S., Kardos, G., Belák, S., and Kiss, I. (2007): Rapid and simultaneous detection of avian influenza and Newcastle disease viruses by duplex polymerase chain reaction assay. Zoonoses Public Health. 54: 38-43.
Fernández J., Fernández-Pacheco P., Rodríguez B., Robles A., Arias M., Sánchez-Vizcaíno J.M. (2006). Development of a conventional and a rapid SybrGreen real-time RT–PCR assays for the detection of African Horse Sickness Virus (AHSV). Proceedings of the 4th International Veterinary Vaccines and Diagnostics Conference. Oslo, Norway, 25-29 June 2006.
Fernández J., Fernández-Pacheco P., Rodríguez B., Robles A., Arias M., Sánchez-Vizcaíno J.M. (2006). Development of a rapid TaqMan real-time RT-PCR assay for the detection of African Horse Sickness Virus (AHSV).Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 214.
Fernández J., Agüero M., Romero L., Sánchez C., Belák S., Arias M., Sánchez-Vizcaíno JM. (2006). Rapid and differential diagnosis of Foot-and-Mouth Disease (FMD), Swine Vesicular Disease (SVD) and Vesicular Stomatitis (VS) by a new multiplex RT-PCR assay, analysing clinical samples. Vet. Res. Accepted.
King DP, Dukes JP, Reid SM, Ebert K, Shaw AE, Boswell L, Mills CE, Ferris NP. (2006). Detection of foot-and-mouth disease virus using field-portable PCR equipment.Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 225.
King DP, Montague N, Ebert K, Reid SM., Dukes JP, Ferris NP, Belsham GJ, Lomonosoff GP. (2006). Development of a novel encapsulated internal control for real-time RT-PCR. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 224.
Kiss I., Germán P., Sámi L., Antal M., Farkas T., Kardos G., Kecskeméti S., Dán Á., Belák S. (2006). Application of LUX (light upon extension) fluorogenic primer utilizing real-time RT-PCR for the rapid detection of avian influenza viruses. Acta Veterinaria Hungarica. 54 (4): 525-533.
Lelli D., Cordioli P., Moreno A., Gamba D., Brocchi E. (2006). Antigen typing elisas for neuraminidase N1, N2, N3 and N7 of avian influenza viruses using monoclonal antibodies. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 194.
McKillen, J., McMenamy M., Hjertner B., Atkins J., McNeilly F., Adair B., Allan G. (2006). Development of a minor groove binder probe assay for sensitive real-time one-step RT-PCR detection of foot-and-mouth disease virus. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 222.
McKillen J., McMenamy M., Hjertner B., Millar A., McNeilly F., Adair B., Allan G. (2006). Sensitive real-time PCR detection of African swine fever virus using a minor groove binder probe assay. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 211.
McKillen J., McMenamy M., Hjertner B., Millar A., McNeilly F., Adair B., Allan G. (2006). Real-time one-step RT-PCR detection of foot-and-mouth disease virus using fluorogenic LUX primers. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 223.
McKillen J., Hjertner B., Millar A., McNeilly F., Belák S., Adair B., Allan G. (2006). Molecular beacon real-time PCR detection of swine viruses. J. Virol. Methods. 2006 Dec 28; [Epub ahead of print]
Moreno A., Lelli D., Sozzi E., Luppi A., Brocchi E., Cordioli P. (2006). Monoclonal antibody-based elisas for the detection of antibodies elicited to H5 and H7 influenza viruses. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 196.
Rasmussen TB., Uttenthal Å., Aguero M. (2006). Detection of three porcine vesicular viruses using multiplex real-time primer-probe energy transfer. J Virol Methods. 134(1-2): 176-82.
Rasmussen TB, Reimann I, Hoffmann B, Depner K, Uttenthal Å, Beer M (2006). Full genome amplification of Border disease virus strain Gifhorn. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 243.
Rodriguez-Sanchez B., Fernández, J; Arias M., Sánchez-Vizcaíno, JM. (2006). Detection of African horse sickness virus serotype 4 by quantitative RT-PCR.Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 213.
Sámi L., Ursu K., McKillen J., Kecskeméti S., Belák S., Kiss I. Simultaneous detection of three porcine viruses by multiplex PCR. Acta Veterinaria Hungarica. Accepted.
Uttenthal Å, Nielsen J, Rasmussen TB (2006). Repeated infections by live non-pathogenic African Swine fever virus (ASFV) does not ease the course of pathogenic ASFV infections in pigs. Proceedings of the 7th International Congress of Veterinary Virology, 24th-27th September 2006, Lisbon, Portugal, p. 257.
Van Reeth K., Labarque G., Pensaert M. (2006). Serological profiles after consecutive experimental infections of pigs with European H1N1, H3N2, and H1N2 swine influenza viruses. Viral Immunology 19: 373-382.

Year 2007
Publications, Abstracts, Proceedings
Antal M, Farkas T, Germán P, Belák S, Kiss I. 2006. Real-time RT-PCR detection of Newcastle disease virus using light upon extension (LUX) fluorogenic primers. J Vet Diagn Investigation. Submitted December, 2006.
Blomström A-L., Hakhverdyan M., Reid SM., Dukes JP., King DP., Belák S., Berg M. (2007). A one-step reverse transcriptase loop-mediated isothermal amplification assay for simple and rapid detection of swine vesicular disease virus. J Virol Methods. Accepted.
Farkas T., Antal M., Sámi L., Germán P., Kecskeméti S., Kardos G., Belák S. and Kiss I. 2007. Rapid and Simultaneous Detection of Avian Influenza and Newcastle Disease Viruses by Duplex Polymerase Chain Reaction Assay. Zoonoses Public Health, 54: 38-43.
King D. P., Montague N., Ebert K., Reid S. M., Dukes J. P., Schädlich L., Belsham G. J., and Lomonossoff G. P. (2007). Development of a novel recombinant encapsidated RNA particle: evaluation as an internal control for diagnostic RT-PCR. J Virol Methods. - Accepted.
Kiss I, Germán P, Sámi L, Antal M, Farkas T, Kardos G, Kecskeméti S, Dán A and S Belák. 2006. Application of real-time RT-PCR utilising LUX (Light Upon eXtension) fluorogenic primer for the rapid detection of avian influenza viruses. Acta Vet Hungarica 54 (4): 525-533.
Nordengrahn A., Gustafsdottir SM., Ebert K., Reid SM., King DP., Ferris NP., Brocchi E., Grazioli S., Landegren U., Merza M. (2007). Evaluation of a novel proximity ligation assay for the sensitive and rapid detection of foot-and-mouth disease virus. Vet Microbiol. Accepted.