1. A central theme of this proposed project was to design, produce, standardise and harmonise internal controls for diagnostic PCR in veterinary virology departments. These controls will set "standards of excellence" for verifiable diagnostic PCR to be used across EU member states in single or multiplex PCR assays.
2. The project attempted further development of real-time, fluorimeter-based PCR technology, utilising sequence-specific fluorescence resonance energy transfer probes (FRET).
3. The project advanced Cleavase/Invander-based technology and developed a multiplex Cleavase/Invader test for detection and differentiation of ASFV/CSFV in a single sample.
4. The project also investigated the possibilities of antibody and nucleic acid multiplex capture enhancement techniques. Novel routes and combinations of coupling the capturing molecules was applied, i.e., anthraquinone-based photo-chemical method.
5. In conclusion, this project applied a multidisciplinary approach by an international consortium to progress and harmonised the technologies involved in the detection of viral nucleic acids in tissue samples and applied these new technologies to the diagnosis of important viral infections.

Photo by SVA's photo section.